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cardiac muscle tissue sheets  (Nikon)


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    Structured Review

    Nikon cardiac muscle tissue sheets
    Cardiac Muscle Tissue Sheets, supplied by Nikon, used in various techniques. Bioz Stars score: 96/100, based on 387 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cardiac+muscle+tissue+sheets/BioStation+IM-Q/pmc04845933-131-5-16
    Average 96 stars, based on 387 article reviews
    cardiac muscle tissue sheets - by Bioz Stars, 2026-09
    96/100 stars

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    Microscopy:

    Article Title: Isoproterenol directs hair follicle-associated pluripotent (HAP) stem cells to differentiate in vitro to cardiac muscle cells which can be induced to form beating heart-muscle tissue sheets
    Article Snippet: The follicles were cultured in DMEM (Sigma-Aldrich, St. Louis, MO) containing 10% fetal bovine serum (FBS), 50 μg/ml gentamycin, 2 mM L -glutamine (GIBCO, Grand Island, NY), 10 mM Hepes (MP Biomedicals, Santa Ana, CA), supplemented with isoproterenol (3 μM) (Sigma-Aldrich) alone, or in combination with bone morphogenetic protein 4 (BMP4) (10 ng /ml ) (HumanZyme, Chicago, IL); activin A (10 ng /ml) (HumanZyme); basic fibroblast growth factor (bFGF) (5 ng /ml) (Millipore, Temecula, CA). .. Beating cardiac muscle cells and cardiac-muscle tissue sheets were visualized and recorded with a BioStation IM-Q (Nikon, Tokyo, Japan) and video microscope camera (ScopPad-500, GelleX, Tokyo, Japan). .. Immunofluorescence staining The cells were incubated with anti-cardiac toroponin T (cTnT) mouse monoclonal antibody (1:250, Gene Tex, Hsinchu City, Taiwan) in blocking buffer (4% BSA, 0.5% Triton X100, 0.04% NaN 3 in PBS) at room temperature for 3 hours.



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    96
    Nikon cardiac muscle tissue sheets
    Cardiac Muscle Tissue Sheets, supplied by Nikon, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cardiac+muscle+tissue+sheets/BioStation+IM-Q/pmc04845933-131-5-16
    Average 96 stars, based on 1 article reviews
    cardiac muscle tissue sheets - by Bioz Stars, 2026-09
    96/100 stars
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